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    微流體阻抗測試

    微流體阻抗測試

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    簡介


      電阻抗測量技術廣泛應用于材料科學、生命科學、食品、疾病診斷等領域?;陔娮杩箼z測的流式細胞儀作為無標記、非侵入式技術而被廣泛的應用于細胞的計數、分選、捕獲、分離及鑒別等。隨著電阻抗測量技術的快速發展,基于電阻抗測量的流式細胞儀正向著快速的、高通量的單細胞分析方向發展。目前,結合熒光細胞分選(fluorescent activated cell sorting,FACS)的熒光標記技術可以快速、準確的實現高通量的細胞分選。但是,FACS技術有兩個主要缺點:一是需要使用標記和抗體對細胞進行修飾,這意味著有可能會改變研究對象;二是FACS設備非常昂貴且操作復雜?;陔娮杩箼z測的微流控技術由于無需對測量對象做標記,也不會侵入到其內部,從而不會對其造成任何破壞。此外,微流控電阻抗檢測技術所用的樣品量較小,而且基于電阻抗檢測的設備易于操作和攜帶。所以,基于電阻抗檢測的微流控技術為細胞檢測提供了一個全新的分析方法。

     

    相關產品


    流體控制模塊

      Fluigent驅動泵有正壓力泵和負壓力泵兩種;

      范圍:正壓力泵的壓力范圍可從0到25、69、345 、800、1000、7000 mbar;負壓力泵的范圍可從0 到 -800 mbar;

      穩定性:正壓泵和負壓泵的壓強穩定性均小于0.1%(測量值),壓力傳感器的分辨率為其量程的0.03%;

      通道個數:多達16個通道的輸出壓力,允許同時進行對16個支路進行液體驅動;

      軟件:MAESFLOTM以及All-in-One軟件,可對16個通道的輸出壓力進行單獨設置。軟件界面可同時顯示輸出壓強的數值和曲線??蓪崿F簡單或復雜波形的壓力驅動,通過SDK軟件開發包,用戶可方便的將MAESFLOTM軟件集成到MATLAB、LabVIEW、Python、C/C++、JAVASCRIPT等軟件中.

      

     

    芯片模塊

      微流體芯片是微流控實驗不可缺少的一個核心組件,而且也是實驗創新的主要部分。對于特定的應用研究,需要采用特定材質及不同通道結構的微流體芯片。目前,電阻抗檢測芯片的是PDMS-玻璃芯片、玻璃-玻璃芯片。采用光刻剝離和磁控濺射技術可將金屬微電極加工在玻璃的表面。

      PDMS電阻抗檢測芯片

      現有PDMS電極芯片的類型有:

      ?“十”字交叉型的微液滴檢測芯片

      “十”字交叉型微液滴檢測芯片可用于常規微液滴的制備與檢測,細胞或病毒包裹的微液滴制備與檢測,無機納米顆粒包裹的微液滴制備與檢測等。

      

     

      ? 溶液混合的檢測芯片

    溶液混合的芯片主要用于研究兩種不同溶液間的擴散程度,不同溶液發生化學反應的過程等。

     

     

      ? 介電譜測量的檢測芯片

      介電譜測量芯片可用于生物細胞(紅細胞、酵母細胞、大腸桿菌、藻細胞等)懸浮液的測量,生物組織/生物大分子溶液的測量,表面活性劑膠束體系的測量以及反膠束與微乳液體系的測量等。

      

     

    玻璃電阻抗檢測芯片

      由于玻璃具有良好的光學透過性,有利于光學顯微鏡觀察玻璃芯片通道的形貌,因此,玻璃電阻抗檢測芯片常被應用于細胞鑒別與計數、微生物種群鑒別、血液檢測等方面。玻璃通道上部和底部的制作的兩對對面電極的電阻抗芯片。

      

     

    流量監控模塊

      Fluigent Flow-Rate Control Module(FRCM)流速反饋控制模塊通過全局控制方式將微流體的流速長時間的維持在所設定的數值上,而驅動泵的輸出壓力則隨儲液池內的液面高度、外界的干擾等因素進行自動調節。因此在儲液池與芯片之間連接Fluigent FLOW UNIT流量計可以實時監測流入到芯片通道內的液體流速,實現恒流控制,并且可以通過改變流速來控制產生微滴的大小以及頻率。

      與任何流體控制器相兼容的FLOW UNIT流量計可測量的液體流速從0到±5 mL/min。FLOW UNIT分為5種不同的型號,每個型號對應一個流速監測范圍??梢愿鶕约旱膶嶒炓筮x擇的流量范圍,所有的測量結果是通過軟件顯示出來,使系統測量的數據一目了然。在內置的水或異丙醇溶液測量的模式下,通過簡單的校準就可以測量與硼硅酸鹽玻璃相兼容的任何流體。此外,體積狹小的FLOW UNIT流量計易于安裝和使用。

      

     

    動態電阻抗檢測模塊

      微流體動態電阻抗檢測模塊可以完成不同測量對象的動態阻抗測量。該模塊主要由Zurich Instruments MFLI鎖相放大器和跨阻差分放大器構成。

      蘇黎世鎖相放大器同時解調一個頻率或多個不同頻率的差分電壓信號,從而給出每一個頻率信號的同相分量和正交分量或者幅值和相位值,同時抵制所有其他頻率信號的干擾。所測量的電阻抗的變化可在本地電腦上的LabOne軟件里的Plotter進行實時觀察且測量的數據可直接保存在本地電腦,方便后續使用MATLAB、Python等軟件進行分析處理。

      

     

    其他輔助設備

      為了觀察微流控芯片通道內的粒子運動或液體的流動情況,需要光學顯微鏡。另一方面還需要不同規格尺寸的毛細導管和轉接頭以便將儲液池、FLOW UNITS及微流控芯片等連接在一起。

    未找到相應參數組,請于后臺屬性模板中添加

     

      

     

    簡介:

      電阻抗測量技術廣泛應用于材料科學、生命科學、診斷等領域?;陔娮杩箼z測的流式細胞儀作為無標記、非侵入式技術而被廣泛的應用于細胞的計數、分選、捕獲、分離及鑒別等。隨著電阻抗測量技術的快速發展,基于電阻抗測量的流式細胞儀正向著快速的、高通量的單細胞分析方向發展。目前,結合熒光激活細胞分選(fluorescent activated cell sorting,FACS)的熒光標記技術可以快速、準確的實現高通量的細胞分選。但是,FACS技術有兩個主要缺點:一是需要使用標記和抗體對細胞進行修飾,這意味著有可能會改變研究對象;二是FACS設備非常昂貴且操作復雜?;陔娮杩箼z測的微流控技術由于無需對測量對象做標記,也不會侵入到其內部,從而不會對其造成任何破壞。此外,微流控電阻抗檢測技術所用的樣品量較小,而且基于電阻抗檢測的設備易于操作和攜帶。所以,基于電阻抗檢測的微流控技術為細胞檢測提供了一個全新的分析方法。

     

     

     

     

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